Orthotopic Ovarian Transplants in Chickens

Orthotopic and heterotopic ovarian tissue transplantation

BACKGROUND Transplantation of ovarian tissue is, at present, the only clinical option available to restore fertility using cryopreserved ovarian tissue. More than 30 transplantations of cryopreserved tissue have been reported, and six babies have been born, worldwide, following this procedure. Despite these encouraging results, it is essential to optimize the procedure by improving the follicul...

Ovarian Tumor-Stroma Interactions in an In Vivo Orthotopic Model

Role of CD4+ T cells in immunobiology of orthotopic corneal transplants in mice.

PURPOSE To determine, with the use of mice genetically deficient in expression of CD4 or CD8 molecules, which T cells are responsible for rejection of orthotopic corneal allografts in mice. METHODS Corneas were prepared from major histocompatibility complex (MHC)-only incompatible, minor histocompatibility (H)- only incompatible, and MHC-plus-minor H incompatible donors and grafted orthotopic...

The technique of orthotopic ovarian transplantation in the chicken.

A surgical technique has been established for orthotopic transplantation of ovarian tissue in newly hatched chickens. In these trials survivability of the chicks after surgical manipulation was 100%. The size and orientation of the ovary at 2 wk of age suggested that the graft was attached and had undergone development. This technique should allow the development of a cryopreservation protocol ...

Screening of the residual normal ovarian tissue adjacent to orthotopic epithelial ovarian carcinomas in nude mice.

The objective of this study was to explore the feasibility and methods of screening the residual normal ovarian tissue adjacent to orthotopic ovarian carcinomas in nude mice. Human epithelial ovarian cancer cells (OVCAR3) were subcutaneously implanted for a tumor source and ovarian orthotopic transplantation. The cancer tissue, proximal paraneoplastic tissue, middle paraneoplastic tissue, remot...